We are seeking a highly motivated research associate to join our efforts in the Biology Department at Fulcrum to participate in building our discovery pipeline for CNS and neuro-muscular diseases. This individual will contribute to scientific efforts focused on building a relationship between the correction of gene expression profiles and functional improvement of disease phenotypes in complex human cellular models.
- Design, develop and execute human iPSC-derived cell culture models of CNS and neuro-muscular diseases to identify functional phenotypes through a variety of physiologically relevant assay techniques. Cell culture models will be assessed utilizing the assay technologies of MEA electrophysiology, seahorse metabolic pathway assays, high-content imaging, fluorescent plate readers, and protein quantification.
- Analyze complex data sets and clearly communicate results to the larger group.
- Actively participate across functional groups and programs to provide knowledge of physiological, biochemical and pharmacologic techniques required to examine target expression, modulation and function.
- Demonstrates understanding of theory and application of cell biology and physiology assays to examine gene expression, subcellular localization, electrophysiological function, fluorescent microscopy techniques and quantitative image analysis.
- BS/MS in biology, molecular and/or cellular biology
- 2-5 years of hands-on experience in neurologic, muscle or cardiovascular diseases
- Knowledge, application and analysis of compound pharmacology testing using in vitro disease models.
- Experience performing and analyzing MEA and/or high definition MEA assays is desired, especially experience with specialized analysis software (i.e. MATLAB, python, or R).
- Skilled in pharmacologic assessment of both small molecules and other therapeutic modalities in primary or stem cell derived cellular systems.
- Proficiency in development of optical and image-based assay approaches and analysis.
- Ability to identify and solve technical issues that arise with equipment, especially electrophysiology equipment.
- Excellent written and oral communication as demonstrated in the quality of the employee’s verbal interactions on projects in a team environment.
- Experience and effectiveness working in a multidisciplinary team environment with common goals and operating in a matrixed environment is essential.
- Rigor, attention to detail and problem-solving skills are required.
- Experience preferred in whole cell patch clamp techniques in either clonal or primary cultured cells, including neurons and myocytes.