Research Associate - Associate Scientist, Biochemistry- Protein Expression and Purification at Neon Therapeutics
Cambridge, MA, US
Position Summary

Neon is seeking a highly motivated Research Associate or Associate to join Neon’s Biochemistry group. The candidate will work within the biochemistry group and as part of an interdisciplinary team to design novel personalized neo-antigen based cancer vaccines. In addition, the candidate will develop new biochemistry technologies for immune monitoring and immunology research.

Job responsibilities include:

Expression of recombinant proteins in bacterial cells (E. coli) and protein purification by affinity, ion-exchange, size-exclusion chromatography. Characterization of protein purity, activity and yield. Develop and optimize expression systems and conditions.
Design and molecular cloning of protein constructs for expression, purification, and assay development (e.g. fusion proteins, mutations).
Perform ELISA and other biochemical assays.
Improvement of current processes and implementation of new technologies.
Analysis and presentation of results to larger scientific groups.
Work productively in a team environment and independently and deliver against timelines.
Keep very well documented electronic lab notebook.

Experience and essential skills:

The candidate must have a BS/MS in biochemistry, biotechnology, biological science and 3+ years of protein biochemistry research experience.
Expert in protein biochemistry and proficiency in various protein purification techniques such as affinity, ion exchange, gel filtration.
Strong experience in protein characterization and bioanalytical techniques such as SDS-PAGE, Western blot, ELISA, SEC required.
Deep understanding of molecular biology and hands-on-experience in molecular cloning (e.g. Gibson assembly)
Familiarity with Äkta purification and HPLC systems is preferred.
Excellent organizational, communication skills and a pro-active mentality.
Excellent attention to detail.
Ability to maintain inventories of equipment, supplies, and related research materials
Ability to effectively work on a team but also independently.